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Wassarman Lab - Publications

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Publications since:


2016

Wassarman, PM, Litscher, ES,:  A bespoke coat for eggs:  Getting ready for fertilization.  Current Topics Dev. Biol., 117, 539-552.

Wassarman, PM (ed.),:  Essays on Dev. Biol., Parts A and B.  Current Topics Dev. Biol., 116, 1-744; 117, 1-710.

 

2015

Litscher, ES, Wassarman, PM,:  A Guide to Zona Pellucida Domain Proteins.  John Wiley and Sons, pp 1-208.

Litscher, E.S. and Wassarman, P.M.:  A Guide to Zona Pellucida Domain Proteins.  John Wiley and Sons, Hoboken, NJ, 258 pp.

 

2014

Litscher, ES, Wassarman, PM,:  Evolution, structure, and synthesis of vertebrate egg-coat proteins.  Trends Dev. Biol., 8, 65-76.

Litscher, E.S. and Wassarman, P.M.:  Evolution, structure, and synthesis of vertebrate egg-coat proteins.  Trends in Developmental Biology 8, 65-76.

Wassarman, P.M.:  Reproductive biology:  Sperm protein finds its mate.  Nature 508, 466-467.

Rosenwaks, Z, Wassarman, P.M. (eds.):  Human Fertility: Methods and Protocols. Methods in Molecular Biology 1154, 1-584.


2013

Wassarman, P.M.:  The zygote.  Brenner's Encyclopedia of Genetics (Malloy, S. and Hughes, K., eds.), 2nd ed., Vol. 3, pp. 400-401, Academic Press.

Wassarman, P.M.:  Mammalian fertilization.  Brenner's Encyclopedia of Genetics  (Malloy, S. and Hughes, K., eds.), 2nd ed., Vol. 3, pp. 36-38, Academic Press.

Wassarman, P.M.:  Becoming a professional scientist at the LMB.  In Memories and Consequences, MRC Laboratory of Molecular Biology, Cambridge (Huxley, H., ed.), Icon Books Ltd. Publ., pp. 189-196. 

Wassarman, PM., Litscher, ES.:  Biogenesis of the mouse egg's extracellular coat, the zona pellucida.  Current Topics Dev. Biol., 102, 243-266.


2012

Wassarman, PM., Litscher, ES.:  Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.  Intl. J. Dev. Biol., 56, 833-839.


2011

Wassarman PM,  The human sperm’s sweet tooth.  Science, 233, 1708-1709.


2010

Plaza, S., Chanut-Delalande, H., Fernandes, I., Wassarman, P.M., and Payre, F.:  From A to Z:  apical structures and zona pellucida-domain proteins.  Trends in Cell Biology 20: 524-532.

Wassarman, P.M. and Litscher, E.S.:.  Egg's ZP3 structure speaks volumes.  Cell 143, 337-338. 

Wassarman, P.M. and Soriano, P.M. (eds.):  Guide to Techniques in Mouse Development, Methods in Enzymology, 2nd edition, Part A, Mice, Embryos, and Cells,  vol. 476, Academic Press, 539 pp.

Wassarman, P.M. and Soriano, P.M. (eds.):  Guide to Techniques in Mouse Development, Methods in Enzymology, 2nd edition, Part B, Mouse Molecular Genetics,  vol. 477, Academic Press, 637 pp.

Litscher, E.S. and Wassarman, P.M.:  Isolation and manipulation of mouse gametes and embryos.  In Guide to Techniques in Mouse Development, Methods in Enzymology (Wassarman, P.M. and Soriano, P., eds.), 2nd edition, Part A, Mice, Embryos, and Cells, 476: 73-85, Academic Press.


2009

Wassarman, P.M. and Litscher, E.S.: The multifunctional zona pellucida and mammalian fertilization. Journal Reproductive Immunology 83: 45-49.

Litscher, E.S., Williams, Z., and Wassarman, P.M.: Zona pellucida glycoprotein ZP3 and fertilization in mammals.  Molecular Reproduction and Development 76: 933-941.

Wassarman, P.M.:  Mammalian fertilization:  The strange case of sperm protein 56.  BioEssays 31: 153-158.


2008

Darie, C.C., Litscher, E.S., and Wassarman, P.M.: Structure, processing, and polymerization of trout egg vitelline envelope proteins. In, Mass Spectrometry in the Life Sciences (Popescu, C., Zamfir, A., and Dinca, N., eds.), pp. 23-36, Springer-Verlag, Heidelberg.

Wassarman, P.M.: Fertilization - Welcome to the fold. Nature 456: 586-587.

Litscher, E.S., Janssen, W.G., Darie, C.C., and Wassarman, P.M.: Purified mouse egg zona pellucida glycoproteins polymerize into homomeric fibrils under non-denaturing conditions. Journal Cellular Physiology 214: 153-157 (2008).

Wassarman, P.M. and Litscher, E.S.: Mammalian fertilization is dependent on multiple membrane fusion events.  In, Cell Fusion: Methods and Protocols 475: 99-113 (Chen, E.H., ed.), Methods in Molecular Biology, Humana Press, Totawa, NJ (2008).

Darie, C.C., Janssen, W.G., Litscher, E.S., and Wassarman, P.M.: Purified trout egg vitelline envelope proteins VEb and VEg polymerize into homomeric fibrils from dimers in vitro. Biochimica Biophysica Acta (Proteins and Proteomics) 1784: 385-392 (2008).

Wassarman, P.M. and Vacquier, V.D. (eds.): Fertilization. International Journal Developmental Biology (Special issue) 52: 409-796 (2008).

Wassarman, P.M. and Litscher, E.S.: Mammalian fertilization: The egg's multifunctional zona pellucida. International Journal Developmental Biology 52: 665-676 (2008).

Wassarman, P.M.:  Zona pellucida glycoproteins. Journal Biological Chemistry 283: 24285-24289 (2008).


2007

Jovine, L., Qi, H., Williams, Z., Litscher, E.S., and Wassarman, P.M.:  Features that affect secretion and assembly of zona pellucida glycoproteins during mammalian oogenesis.  In, Gamete Biology (S. Gupta K. Koyama, and J.F. Murray, eds.), Nottingham Univ. Press, pp. 187-202 (2007).

Kohn, M.J., Kaneko, K.J., Yagi, R., Litscher, E.S., Wassarman, P.M., DePamphilis, M.L.:  Dickkopf-like 1- a protein unique to mammals that is associated both with formation of trophoblast stem cells and with spermatogenesis. In, Y Chromosome and Male Germ Cell Biology in Health and Diseases (Lau, Y.-F.C. and Chan, W.Y., eds.), World Scientific Publishers, Hackensack, NJ, pp. 185-199 (2007).


2006

Williams, Z., Litscher, E.S., Jovine, L., and Wassarman, P.M.: Polypeptide encoded by mouse ZP3 exon-7 is necessary and sufficient for sperm binding in vitro Journal Cellular Physiology 207: 30-39 (2006).

Jovine, L., Janssen, W.G., Litscher, E.S., and Wassarman, P.M.: The PLAC1-homology region of the ZP domain is sufficient for protein polymerization.  BMC Biochemistry 7: 11 (9 pp.) (2006).


2005

Jovine, L., Darie, C.C., Litscher, E.S., and Wassarman, P.M.: Zona pellucida domain proteins. Annual Review Biochemistry 74: 83-114 (2005).

Wassarman, P.M., Jovine, L., Qi, H., Williams, Z., Darie, C., and Litscher, E.S.: Recent aspects of mammalian fertilization research.  Molecular Cellular Endocrinology  234: 95-103 (2005).

Wassarman, P.M.:  Contribution of mouse egg zona pellucida glycoproteins to gamete recognition during fertilization.  Journal Cellular Physiology 204: 388-391 (2005).

Darie, C.C., Biniossek, M.L., Thumfart, J.O., Gawinowicz, M.A., Milgrom, Y., Jovine, L., Litscher, E.S., and Wassarman, P.M.:  Mass spectrometric evidence that proteolytic processing of rainbow trout egg vitelline envelope proteins takes place on the egg.  Journal Biological Chemistry 280: 37585-37598 (2005).


2004

Jovine, L., Qi, H., Williams, Z., Litscher, E.S., and Wassarman, P.M.:  A duplicated motif controls assembly of zona pellucida domain proteins.  Proceedings National Academy Sciences, USA  101: 5922-5927 (2004).

Darie, C.C., Biniossek, M.L., Jovine, L., Litscher, E.S., and Wassarman, P.M.:  Structural characterization of fish egg vitelline envelope proteins by mass spectrometry.  Biochemistry 43: 7459-7478 (2004).


2003

Wassarman, P.M. and Keller, G. (eds.):  Differentiation of embryonic stem cells.  Methods in Enzymology, Vol. 365, Academic Press, San Diego, CA, 535 pp. (2003).


2002

Qi, H., Williams, Z.,  and Wassarman, P.M.:  Secretion and assembly of zona pellucida glycoproteins by growing mouse oocytes microinjected with epitope-tagged cDNAs for mZP2 and mZP3.  Molecular Biology Cell 13:  530-541 (2002).

Wassarman, P.M.:  Channels of communication in the ovary.  Nature Cell Biology 4 (S1): S7-S9 (2002).

Jovine, L., Qi, H., Williams, Z., Litscher, E., and Wassarman, P.M.:  The ZP domain is a conserved module for protein polymerization. Nature Cell Biology 4:  457-461 (2002). 

Jovine, L., Park, J., and Wassarman, P.M.:  Sequence similarity between stereocilin and otoancorin points to a unified mechanism for mechanotransduction of sound in the inner ear.  BMC Cell Biology 3:  28 (2002).


2001

Williams, Z. and Wassarman, P.M.:  Secretion of mouse ZP3, the sperm receptor, requires cleavage of its polypeptide at a consensus furin cleavage site.  Biochemistry 40:  929-937 (2001).

Wassarman, P.M., Jovine, L., and Litscher, E.S.:  A profile of fertilization in mammals.  Nature Cell Biology 3: E59-E64 (2001).

Cohen, N. and Wassarman, P.M.:  Association of egg zona pellucida glycoprotein mZP3 with sperm protein sp56 during fertilization in mice. International Journal Developmental Biology 45: 569-576 (2001).


1999

Wassarman, P.M.:  Mammalian fertilization:  Molecular aspects of gamete adhesion, exocytosis, and fusion.  Cell 96: 175-183 (1999).

Wassarman, P.M. and Wolffe, A.P. (eds.), Chromatin, Methods in Enzymology, Vol. 304, Academic Press, San Diego, CA, 815 pp.  (1999).

Qi, H. and Wassarman, P.M.:  Secretion of zona pellucida glycoprotein mZP2 by growing oocytes from mZP3+/+ and mZP3-/- mice.  Developmental Genetics 25: 95-102 (1999).

Litscher, E.S., Qi, H., and Wassarman, P.M.:  Mouse zona pellucida glycoproteins mZP2 and mZP3 undergo carboxy-terminal proteolytic processing in growing oocytes.  Biochemistry 38:  12280-12287 (1999).

Litscher, E.S., Liu, C., Echelard, Y., and Wassarman, P.M.:  Zona pellucida glycoprotein mZP3 produced in milk of transgenic mice is active as a sperm receptor, but can be lethal to newborns.  Transgenic Research 8:  361-369 (1999).


1998

Chen, J., Litscher, E.S., and Wassarman, P.M.:  Inactivation of the mouse sperm receptor, mZP3, by site-directed mutagenesis of individual serine residues located at the combining site for sperm.  Proceedings National Academy of Sciences, USA 95:  6193-6197 (1998).


1997

Liu, C., Litscher, E.S, and Wassarman, P.M.:  Zona pellucida glycoprotein mZP3 bioactivity is not dependent on the extent of glycosylation of its polypeptide or on sulfation and sialylation of its oligosaccharides.  Journal Cell Science 110:  745-752 (1997).

Wassarman, P.M., Qi, H., and Litscher, E.S.:  Mutant female mice carrying a single mZP3 allele produce eggs with a thin zona pellucida, but reproduce normally. Proceedings Royal Society, Biological Sciences 264:  323-328 (1997).


1996

Litscher, E.S. and Wassarman, P.M.:  Characterization of a mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro.  Biochemistry  35:  3980-3986 (1996).

Liu, C., Litscher, E.S., Mortillo, S., Sakai, Y., Kinloch, R.A., Stewart, C.L., and Wassarman, P.M.:  Targeted disruption of  the mZP3 gene results in production of eggs lacking a zona pellucida and infertility in female mice.  Proceedings National Academy of Sciences, USA 93:  5431-5436 (1996).

Wassarman, P.M., Liu, C., and Litscher, E.S.:  Constructing the mouse egg zona pellucida:  some new pieces of an old puzzle.  Journal Cell Science 109:  2001-2004 (1996).

Litscher, E.S. and Wassarman, P.M.:  Recombinant hamster sperm receptors that exhibit species-specific binding to sperm.  Zygote  4:  229-236 (1996).


1995

Kinloch, R.A., Sakai, Y., and Wassarman, P.M.:   Mapping the mouse ZP3 combining site for sperm by exon swapping and site-directed mutagenesis.  Proceedings National Academy of Sciences, USA 92:  263-267  (1995).

Litscher, E.S, Juntunen, K., Seppo, A., Niemalä, R., Penttilä, L., Renkonen, O., and Wassarman, P.M.:   Oligosaccharide constructs with defined structures that inhibit binding of mouse sperm to unfertilized eggs in vitro.   Biochemistry  34:  4662-4669 (1995).

Liu, C., Litscher, E.S., and Wassarman, P.M.:  Transgenic mice that reproduce normally despite reduced numbers of functional sperm receptors on their eggs.  Molecular Biology Cell 6:  577-585 (1995).

Wassarman, P.M. and Litscher, E.S.:  Sperm-egg recognition mechanisms in mammals.  In,  Current Topics in Developmental Biology, Vol. 30 (R.A. Pedersen and G. Schatten, eds.), Academic Press, Orlando, FL,  pp. 1-19 (1995).

Wassarman, P.M.:  Towards molecular mechanisms for gamete adhesion and fusion during mammalian fertilization.  Current Opinion Cell Biology 7: 658-664  (1995).


1994

Wassarman, P.M. and Albertini, D.F.:  Cellular and molecular biology of the mammalian ovum.  In,  The Physiology of Reproduction  (E. Knobil and J.D. Neill, eds.), Vol. 1, 2nd ed., Raven Press, NY, pp. 79-122  (1994).

Cheng, A., Le, T., Palacios, M., Bookbinder, L.H., Wassarman, P.M., F. Suzuki, and Bleil, J.D.:  Sperm-egg recognition in the mouse:  Characterization of sp56, a sperm protein having specific affinity for ZP3.  Journal Cell Biology  125:  867-878 (1994).


1993

Wassarman, P.M.:  Mammalian fertilization: Sperm receptor genes and glycoproteins.  Advances Developmental Biochemistry  2:  155-199 (1993).

Wassarman, P.M.:  Mammalian eggs, sperm, and fertilization:  Dissimilar cells with a common goal.  Seminars Developmental Biology 4:  189-197 (1993).

Wassarman, P.M. and DePamphilis, M.L. (eds.): Guide to Techniques in Mouse Development, Methods in Enzymology, Vol. 225, Academic Press, Orlando, FL, 1021 pp. (1993).

Lira, S.A., Schickler, M., and Wassarman, P.M.:  Cis-acting DNA elements involved in oocyte-specific expression of mouse sperm receptor gene mZP3 are located close to the gene's transcription start-site.  Molecular Reproduction Development 36: 494-499  (1993).

Richards, W.G., Carroll, P.M., Kinloch, R.A., Wassarman, P.M., and Strickland, S.:  Creating maternal effect mutations in transgenic mice:  Antisense inhibition of a specific oocyte gene product.  Developmental Biology  160:  543-553  (1993).


1992

Schickler, M., Lira, S.A., Kinloch, R.A., and Wassarman, P.M.:  A mouse oocyte-specific protein that binds to a region of mZP3 promoter involved in regulating oocyte-specific expression of the mZP3 gene.  Molecular Cellular Biology 12: 120-127 (1992).

Kinloch, R.A., Mortillo, S., and Wassarman, P.M.:  Transgenic mouse eggs with functional hamster sperm receptors in their zona pellucida.  Development 115:  937-946 (1992).

Rosiere, T.K. and Wassarman, P.M.:  Identification of a region of mouse zona pellucida glycoprotein mZP3 that possesses sperm receptor activity.  Developmental Biology 154:  309-317 (1992).


1991

Mortillo, S. and Wassarman, P.M.:  Differential binding of gold-labeled zona pellucida glycoproteins mZP2 and mZP3 to mouse sperm membrane compartments.  Development 113:  141-149 (1991).

Kinloch, R.A., Mortillo, S., Stewart, C.L., and Wassarman, P.M.:  Embryonal carcinoma cells transfected with ZP3 genes differentially glycosylate similar polypeptides and secrete active mouse sperm receptor.  Journal Cell Biology  115:  655-664 (1991).


1990

Wassarman, P.M.:  Profile of a mammalian sperm receptor.  Development  108:  1-19 (1990).

Moller, C.C., Bleil, J.D., Kinloch, R.A., and Wassarman, P.M.:  Structural and functional relationships between mouse and hamster zona pellucida glycoproteins.  Developmental Biology 137: 276-286 (1990).

Bleil, J.D. and Wassarman, P.M.:  Identification of a ZP3-binding protein on acrosome-intact mouse sperm by photoaffinity crosslinking.  Proceedings National Academy of Sciences, USA  87: 5563-5567 (1990).

Lira, S.A., Kinloch, R.A., Mortillo, S., and Wassarman, P.M.:  An upstream  region of the mouse ZP3 gene directs expression of firefly luciferase specifically  to growing oocytes in transgenic mice.  Proceedings National Academy of Sciences, USA  87: 7215-7219 (1990).

Kinloch, R.A., Ruiz-Seiler, B., and Wassarman, P.M.:  Genomic organization  and polypeptide primary structure of zona pellucida glycoprotein hZP3, the, hamster sperm receptor.  Developmental Biology  142: 414-421 (1990).


1989

Wassarman, P.M.:  Role of carbohydrates in receptor-mediated fertilization in mammals.  In, Carbohydrate Recognition in Cellular Function (Bock, G. and Harnett, S., eds.), Ciba Foundation Symposia (No. 145), John Wiley and Sons, Chichester, pp. 135-155 (1989).

Moller, C.C. and Wassarman, P.M.:  Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs.  Developmental Biology  132: 103-112 (1989).

Wassarman, P.M. and Kornberg, R.D. (eds.), Nucleosomes, Methods in Enzymology, Vol. 170, Academic Press, Orlando, FL, 683 pp. (1989).

Vazquez, M.H., Phillips, D.M., and Wassarman, P.M.:  Interaction of mouse sperm  with purified sperm  receptors covalently-linked to silica beads. Journal Cell Science  92:  713-722 (1989).

Roller, R.J., Kinloch, R.A., Hiraoka, B.Y., Li, S.S.-L., and Wassarman, P.M.: Gene expression during mammalian oogenesis and early embryogenesis:  Quantification of three messenger-RNAs abundant in fully-grown mouse  oocytes.  Development  106:  251-261 (1989).

Brown, J., Cebra-Thomas, J.A., Bleil, J.D., Wassarman, P.M., and Silver, L.M.:  A premature acrosome reaction is programmed by mouse t haplotype during  sperm differentiation and could play a role in transmission ratio distortion.  Development  106:  769-773 (1989).


1988

Wassarman, P.M.:  The mammalian ovum.  In, The Physiology of Reproduction (E. Knobil and J.D. Neill, eds.), Vol. 1, Raven Press, NY, pp. 69-102 (1988).

Wassarman, P.M.:  Zona pellucida glycoproteins.  Annual Review Biochemistry 57:  415-442 (1988).

Wassarman, P.M.:  Fertilization in mammals.  Scientific American 255:  78-84 (December, 1988).

Bleil, J.D., Greve, J.M., and Wassarman, P.M.:  Identification of a secondary sperm receptor in the mouse egg zona pellucida:  Role in maintenance of binding of acrosome-reacted sperm to eggs.  Developmental Biology 128:  376-385 (1988).

Kinloch, R.A., Roller, R.J., Fimiani, C.M., Wassarman, D.A., and Wassarman, P.M.:  Primary structure of the mouse sperm receptor's polypeptide chain determined by genomic cloning.  Proceedings National Academy of Sciences, USA 85:  6409-6413 (1988).

Bleil, J.D. and Wassarman, P.M.:  Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity.  Proceedings National Academy of Sciences, USA  85:  6778-6782 (1988).


1987

Wassarman, P.M.:  The biology and chemistry of fertilization.  Science  235:  553-560 (1987).

Wassarman, P.M.:  Early events in mammalian fertilization.  Annual Review Cell Biology  3:  109-142 (1987).

Wassarman, P.M.:  The zona pellucida:  A coat of many colors.  BioEssays  6:  161 (1987) [cover image].

DePamphilis, M.L. and Wassarman, P.M.:  Regulation of chromosomal replication and transcription during early mammalian development.  BioEssays  6:  265 (1987).


1986

Perona, R.M. and Wassarman, P.M.:  Mouse blastocysts hatch in vitro by using a trypsin-like proteinase associated with cells of mural trophectoderm.  Developmental Biology 114:  42-52 (1986).

Bleil, J.D. and Wassarman, P.M.:  Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm.  Journal Cell Biology 102:  1363-1371 (1986).


1985

Greve, J.M. and Wassarman, P.M.:  Mouse egg extracellular coat is a matrix of interconnected filaments possessing a structural repeat.  Journal Molecular Biology 181:  253-264 (1985).

Florman, H.M. and Wassarman, P.M.:  O-Linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity.  Cell 41:  313-324 (1985).

Wassarman, P.M., Bleil, J.D., Florman, H.M., Greve, J.M., Roller, R.J., Salzmann, G.S., and Samuels, F.G.:  The mouse egg's sperm receptor:  What is it and how does it work?  In, Molecular Biology of Development, Cold Spring Harbor Symposium Quantitative Biology  50:  11-18 (1985).