labslabs.htmlshapeimage_1_link_0
home.html
http://icahn.mssm.edu/departments-and-institutes/developmental-and-regenerative-biology
homehome.htmlshapeimage_4_link_0
facilitiesfacilities.htmlshapeimage_5_link_0
seminarsseminars.htmlshapeimage_6_link_0
linkslinks.htmlshapeimage_7_link_0
lemischka:moore
peoplelmlab.htmlshapeimage_9_link_0
publicationslmpub.htmlshapeimage_10_link_0
funlmlink.htmlshapeimage_11_link_0
 

Lemischka:Moore Lab - Research


Welcome to the
Lemischka:Moore Laboratory
for Integrative Stem Cell Biology

Ihor Lemischka

   The focus of our research is to understand the molecular and cellular nature of the undifferentiated stem cell "states", and how such states are altered during a change in cell fate. The underlying rationale for our studies is that the complement of gene-products and their inter-relationships that exist in stem cells accounts for their remarkable abilities to balance self-renewal and differentiation decision processes. We study both adult and embryonic stem (ES) cells, primarily from the mouse, but also from the human. As a first step, we have comprehensively identified most, if not all gene-products that are expressed in highly purified hematopoietic stem cell (HSC). We performed similar analyses in mouse ES cells. Such molecular "signatures" provide parts-lists that are available to the stem cells. The challenge has been to functionally address the roles that these molecules play in mediating the biological properties of HSC and ES cells. Further, we would like to understand how these molecular components are "wired" into regulatory signaling and transcriptional networks.

   To explore these issues we have utilized a number of global gene-expression perturbation technologies, such as inhibitory short hairpin RNA (shRNA). We have successfully down regulated the expression levels of candidate regulatory molecules in both HSC and ES cells. A number of these play crucial regulatory roles in processes such as self-renewal, proliferation, and differentiation. We have further developed strategies that allow the analyses of cell-fate change dynamics at multiple biochemical and molecular levels in response to defined and precisely controlled changes in the expression levels of key regulatory molecules. These strategies have provided the first in-depth view of how a cell-fate decision actually occurs at the transcriptional, post-transcriptional, translational, and post-translational levels. An important aspect of our overall efforts is computational and quantitative analyses. We anticipate that our approach will yield a systems biology level description and understanding of stem cell decision processes. This in turn, will have profound implications in future efforts focused on applying basic stem cell research in translational as well as clinical contexts.

Kateri Moore

      My research is focused on defining the cellular and molecular nature of hematopoietic stem cells (HSCs) and the molecular cross-talk that occurs between stem cells and their niche. We use transgenic mouse models to study HSC during normal homeostasis and after stress. We are interested in the basic property of self-renewal and whether HSCs really do self-renew to full functional potential when they undergo cell division. The lab is also using specific factors to directly induce hemogenesis from somatic cells, both mouse and human. We have shown that the induction process recapitulates developmental hematopoies and molecular cues from these studies have led to a new investigation of HSC precursor populations during embryonic development. Overall our studies will have broad implications on the behavior of all stem cells including those that contribute to metastatic cancer and leukemias. 

 
lab info



Ihor Lemischka
PROFESSOR
ihor.lemischka@mssm.edu

212-659-8265 (office / lab)
212-803-6740 (fax)



Kateri Moore
ASSOCIATE PROFESSOR
kateri.moore@mssm.edu

212-659-8265 (office / lab)
212-803-6740 (fax)

lab members:
research faculty & scientists 
Cundiff, Paige
Papatsenko, Dmitri
Zhou, Hongwei

postdoc:
Brosh, Ran
Darr, Henia
Lee, Dung-Fang
Pereira, Filipe

graduate student:
Bernitz, Jeffrey
Chang, Betty
Gomes, Andreia
Katz, Suzanna
Kim, Huen Suk
Lau, Kwun Kit
Yuan, Ye (James)
Waghray, Avinash

stem cell core:
D'Souza, Sunita
Alexeeva, Vera
Rizvi, Hira

research staff:
Hrynyk, Iryna
Liu, Ying
Niu, Xiaohong

see photos and more here.
mailto:ihor.lemischka@mssm.edu?subject=mailto:kateri.moore@mssm.edu?subject=shapeimage_13_link_0shapeimage_13_link_1shapeimage_13_link_2shapeimage_13_link_3shapeimage_13_link_4shapeimage_13_link_5shapeimage_13_link_6shapeimage_13_link_7shapeimage_13_link_8shapeimage_13_link_9shapeimage_13_link_10shapeimage_13_link_11shapeimage_13_link_12shapeimage_13_link_13shapeimage_13_link_14shapeimage_13_link_15shapeimage_13_link_16shapeimage_13_link_17shapeimage_13_link_18shapeimage_13_link_19shapeimage_13_link_20shapeimage_13_link_21shapeimage_13_link_22shapeimage_13_link_23shapeimage_13_link_24shapeimage_13_link_25shapeimage_13_link_26shapeimage_13_link_27shapeimage_13_link_28shapeimage_13_link_29shapeimage_13_link_30shapeimage_13_link_31shapeimage_13_link_32
key publications

2013
Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, Lemischka IR, Moore K.,
Induction of a hemogenic program in mouse fibroblasts.
Cell Stem Cell. J Aug 1;13(2):205-18. doi: 10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.  PMID: 23770078
2012
Lee DF, Su J, Ang YS, Carvajal-Vergara X, Mulero-Navarro S, Pereira CF, Gingold J, Wang HL, Zhao R, Sevilla A, Darr H, Williamson AJ, Chang B, Niu X, Aguilo F, Flores ER, Sher YP, Hung MC, Whetton AD, Gelb BD, Moore KA, Snoeck HW, Ma'ayan A, Schaniel C, Lemischka IR..,
Regulation of embryonic and induced pluripotency by aurora kinase-p53 signaling.
Cell Stem Cell. J Aug 3;11(2):179-94. doi: 10.1016/j.stem.2012.05.020.  PMID: 22862944

see more publications here.lmpub.htmlshapeimage_15_link_0
3labs.html

Induction of a hemogenic program in mouse fibroblasts. Pereria et al.

Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.  PMID: 23770078