facilitieszf

Zebrafish Shared Research Facility

location:

Annenberg Building

1468 Madison Avenue

25th Floor, Room 220 & 29

New York, NY 10029

contacts:

Kirsten Sadler Edepli, Director
kirsten.edepli@mssm.edu

tel: 212-241-7152

Evan Closser, Manager
evan.closser@mssm.edu

tel: 212-241-0227

Overview

Zebrafish are a well-established vertebrate model for developmental studies and gene discovery and are emerging as a powerful system to model human disease. Their embryos are transparent and rapidly develop outside of the mother enabling detailed observation of developmental processes in real time using a dissecting microscope. The average clutch size is ~100 embryos, over 80% of which develop into mature animals in 5 days. The high genetic conservation between zebrafish and humans and the similarities between mammalian and zebrafish organ development and function enables not only the elegant developmental studies but also studies of disease pathways and processes.

The Zebrafish Facility is directed by Dr. Kirsten Sadler Edepli and provides Mount Sinai researchers with an array of services:

•consultation with the facility director and manager to aid with experimental design and interpretation of results
•samples for pilot experiments, free of charge
•morpholino injection to deplete embryos of a gene of interest
•RNA injection for over expression in embryos
•importing mutant or transgenic lines upon request
•library of current mutant and transgenic stocks
•protocols for working with zebrafish embryos
•training investigators to carry out and analyze data using zebrafish